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翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Heidenhain's AZAN trichrome stain ： ウィキペディア英語版 Heidenhain's AZAN trichrome stain Trichrome stains are staining methods in which three anionic dyes are used, in conjunction with either phosphomolybdic acid (PMA), phosphotungstic acid (PTA), or a mixture of these heteropolyacids. Probably the first trichrome method was that of Frank B Mallory, an American pathologist, first published in 1900.〔Mallory FB (1900) A contribution to staining methods. Journal of Experimental Medicine 5: 15-20.〕 Unfortunately, none of Mallory's publications (they go from 1891〔Mallory FB (1891) Phospho-molybdic acid-haematoxylin. Anatomischer Anzeiger 6: 375-376.〕 to 1938〔Mallory FB (1938) Pathological Technique. New York: Hafner.〕) provide any explanation of the rationales of either his trichrome or his phosphotungstic acid-haematoxylin (PTAH) method. Nobody knows why Mallory introduced heteropolyacids into microtechnique. Mallory's trichrome method, using acid fuchsine followed by a solution containing PTA, orange G and aniline blue, provides dark red nuclei, orange erythrocytes, and blue collagen fibres, cartilage matrix and mucus.〔Kiernan JA (2008) Histological and Histochemical Methods. Theory and Practice. 4th ed. Bloxham, UK: Scion. ISBN 978-1-904842-42-2〕 In 1915, M. Heidenhain introduced azocarmine G in place of the acid fuchsine of Mallory's method. Heidenhain also introduced visually controlled destaining to provide for different colours in cell nuclei (dark red), collagen (blue) and a variety of colours in cytoplasm. ==References== 〔 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Heidenhain's AZAN trichrome stain」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.